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KMID : 0982820080070010022
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Journal of Lung Cancer
2008 Volume.7 No. 1 p.22 ~ p.24
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Mutational Analysis of the Tumor Suppressor WTX Gene in Non-small Cell Lung Cancer
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Moon Seok-Whan
Chung Yeun-Jun
Yoo Nam-Jin
Kim Min-Sung
Lee Sug-Hyung
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Abstract
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Purpose: In a recent study of Wilms’ tumors, a new X chromosome gene, Wilms’ tumor gene on the X chromosome (WTX), was discovered that was found to harbor small deletions and point mutations. The WTX protein negatively regulates Wnt/?-catenin signaling, and is considered to be a tumor suppressor gene. One of the questions about the WTX gene is whether the genetic alterations of the WTX gene are specific only to Wilms’ tumors. The aim of this study was to explore whether the WTX gene mutation is a characteristic of human non-small cell lung cancer (NSCLC).
Materials and Methods: In the current study, we analyzed the part of the WTX gene encoding the N-terminal of WTX, where most of the WTX point mutations have been detected in Wilms’ tumors. Forty-eight NSCLC tissues were analyzed by a single-strand conformation polymorphism assay and DNA sequencing.
Results: SSCP analysis revealed no evidence of somatic mutations in the DNA sequences encoding the N-terminal of the WTX gene in the 48 NSCLC tissues.
Conclusion: The data presented here indicate that the WTX gene may not be somatically-mutated in human NSCLCs, and suggest that NSCLCs may not utilize mutational events of the WTX gene in the process of pathogenesis.
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KEYWORD
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Non-small cell lung cancer, WTX, Wnt, Mutation
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